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Genome-Wide Library ScreeningÂ
Genome-wide library screening is a technique used to identify genes that are involved in a specific biological process or phenotype. This technique involves the use of a library of DNA sequences, such as a genome-wide cDNA or RNAi library, to screen for genes that affect the phenotype of interest. Here is a general overview of…
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Generation of Stable Cell Lines
Stable cell lines are used in many applications, including protein production, drug screening, and functional studies. The generation of stable cell lines involves the introduction of a foreign gene of interest into the host cell genome and the selection and expansion of cells that stably express the gene. Here is a general overview of the…
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Stem Cells Isolation
Stem cells are a type of cell that can differentiate into different types of cells in the body. They are of great interest in research and medical applications because of their potential to regenerate damaged tissues and organs. The isolation of stem cells involves several steps, depending on the type of stem cell and the…
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Liposome Formulation
Liposomes are microscopic spherical vesicles composed of a lipid bilayer that can encapsulate a variety of substances, including drugs, peptides, and nucleic acids. Liposomes have gained significant attention in drug delivery due to their biocompatibility, ability to protect cargo molecules from degradation, and ability to target specific cells or tissues. The formulation of liposomes involves…
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The Global Life Science
The global life science industry includes a wide range of businesses and organizations involved in the research, development, manufacturing, and commercialization of products and services related to the biological sciences. This can include pharmaceuticals, medical devices, biotechnology, diagnostics, agriculture, and environmental sciences, among others. The global life science industry is a major contributor to the…
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The Host Cell Genome
The host cell genome refers to the complete set of genetic material (DNA) that is present within the cells of a particular organism or cell line. The genome contains all of the instructions that are necessary for the cell to function and reproduce, including the genes that encode for proteins, as well as non-coding regions…
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Clonal Cell Line Selection
Clonal cell line selection is the process of isolating a single, genetically homogeneous population of cells from a mixed population of cells. This is typically done to establish a stable cell line that expresses a specific gene or phenotype of interest. The process of clonal cell line selection typically involves plating the mixed population of…
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RNAi-Encoding Construct
An RNAi-encoding construct is a type of genetic construct that is designed to produce small interfering RNA (siRNA) molecules within a cell. RNA interference (RNAi) is a natural cellular process that allows cells to regulate gene expression by targeting specific messenger RNA (mRNA) molecules for degradation. RNAi-encoding constructs are often used in genetic research to…
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Express Gene
Expressing a gene involves using the cellular machinery to produce the protein that is encoded by the gene. The process typically involves transcribing the DNA sequence of the gene into an RNA molecule, which is then translated into a protein. The first step in expressing a gene is to introduce the DNA sequence into the…
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Transform Customer-Provided Custom Cell Line
Transforming a customer-provided custom cell line typically involves introducing a foreign DNA sequence, such as a plasmid, into the cells to modify their genetic makeup. This can be accomplished using a variety of methods, including transfection, electroporation, or viral transduction. The specific method used will depend on the type of cell line, the desired level…