An RNAi-encoding construct is a type of genetic construct that is designed to produce small interfering RNA (siRNA) molecules within a cell. RNA interference (RNAi) is a natural cellular process that allows cells to regulate gene expression by targeting specific messenger RNA (mRNA) molecules for degradation. RNAi-encoding constructs are often used in genetic research to selectively knock down the expression of specific genes.
An RNAi-encoding construct typically consists of a promoter sequence, which drives the expression of the RNAi molecule, and a DNA sequence that encodes the siRNA. The siRNA sequence is designed to be complementary to the mRNA sequence of the target gene, which allows it to bind to the mRNA molecule and trigger its degradation.
When the RNAi-encoding construct is introduced into cells, it is transcribed into an RNA molecule, which is then processed into the siRNA. The siRNA then binds to the mRNA of the target gene, leading to its degradation and a reduction in the expression of the target protein.
RNAi-encoding constructs can be introduced into cells using a variety of methods, such as transfection, electroporation, or viral transduction. They are often used in combination with other genetic constructs, such as reporter genes or expression vectors, to study the function of specific genes in a cellular or organismal context.