Author: Bob Gog

  • Liposome Formulation

    Liposomes are microscopic spherical vesicles composed of a lipid bilayer that can encapsulate a variety of substances, including drugs, peptides, and nucleic acids. Liposomes have gained significant attention in drug delivery due to their biocompatibility, ability to protect cargo molecules from degradation, and ability to target specific cells or tissues. The formulation of liposomes involves…

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  • The Global Life Science

    The global life science industry includes a wide range of businesses and organizations involved in the research, development, manufacturing, and commercialization of products and services related to the biological sciences. This can include pharmaceuticals, medical devices, biotechnology, diagnostics, agriculture, and environmental sciences, among others. The global life science industry is a major contributor to the…

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  • The Host Cell Genome

    The host cell genome refers to the complete set of genetic material (DNA) that is present within the cells of a particular organism or cell line. The genome contains all of the instructions that are necessary for the cell to function and reproduce, including the genes that encode for proteins, as well as non-coding regions…

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  • Clonal Cell Line Selection

    Clonal cell line selection is the process of isolating a single, genetically homogeneous population of cells from a mixed population of cells. This is typically done to establish a stable cell line that expresses a specific gene or phenotype of interest. The process of clonal cell line selection typically involves plating the mixed population of…

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  • RNAi-Encoding Construct

    An RNAi-encoding construct is a type of genetic construct that is designed to produce small interfering RNA (siRNA) molecules within a cell. RNA interference (RNAi) is a natural cellular process that allows cells to regulate gene expression by targeting specific messenger RNA (mRNA) molecules for degradation. RNAi-encoding constructs are often used in genetic research to…

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  • Express Gene

    Expressing a gene involves using the cellular machinery to produce the protein that is encoded by the gene. The process typically involves transcribing the DNA sequence of the gene into an RNA molecule, which is then translated into a protein. The first step in expressing a gene is to introduce the DNA sequence into the…

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  • Transform Customer-Provided Custom Cell Line

    Transforming a customer-provided custom cell line typically involves introducing a foreign DNA sequence, such as a plasmid, into the cells to modify their genetic makeup. This can be accomplished using a variety of methods, including transfection, electroporation, or viral transduction. The specific method used will depend on the type of cell line, the desired level…

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  • Luciferase, GFP, RFP, and YFP

    Luciferase, GFP, RFP, and YFP are all molecular tools commonly used in biological research. Luciferase is an enzyme that can produce light when it interacts with certain chemicals, such as luciferin. It is often used as a reporter gene in bioluminescence assays to monitor gene expression or protein activity in live cells or animals. GFP,…

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  • Stable Cell Lines

    Stable cell lines are a population of cells that have been genetically modified to express a gene of interest in a stable and heritable manner. These cell lines are commonly used in molecular biology and drug discovery to study gene function, screen for potential drug targets, and test the efficacy of potential therapeutics. The process…

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  • Plasmid DNA Construct Cloning

    Plasmid DNA construct cloning is a widely used technique in molecular biology that involves the insertion of a DNA fragment of interest into a plasmid vector. The plasmid vector is then used to propagate and amplify the DNA fragment of interest for various downstream applications such as protein expression, RNA transcription, and gene editing. The…

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