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Luciferase, GFP, RFP, and YFP
Luciferase, GFP, RFP, and YFP are all molecular tools commonly used in biological research. Luciferase is an enzyme that can produce light when it interacts with certain chemicals, such as luciferin. It is often used as a reporter gene in bioluminescence assays to monitor gene expression or protein activity in live cells or animals. GFP,…
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Stable Cell Lines
Stable cell lines are a population of cells that have been genetically modified to express a gene of interest in a stable and heritable manner. These cell lines are commonly used in molecular biology and drug discovery to study gene function, screen for potential drug targets, and test the efficacy of potential therapeutics. The process…
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Plasmid DNA Construct Cloning
Plasmid DNA construct cloning is a widely used technique in molecular biology that involves the insertion of a DNA fragment of interest into a plasmid vector. The plasmid vector is then used to propagate and amplify the DNA fragment of interest for various downstream applications such as protein expression, RNA transcription, and gene editing. The…
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Generation of Stable Cell Lines
Generation of stable cell lines is a commonly used method in biological research and drug discovery to create cell models that stably express a specific gene or protein of interest. Stable cell lines are generated by introducing a DNA construct containing the gene or protein of interest into a host cell line, followed by selection…
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Animal RNAi Studies
Animal RNAi studies are experimental studies where RNA interference (RNAi) is used to specifically target and knockdown the expression of genes in animal models. RNAi is a powerful tool for studying gene function, as it allows for the rapid and specific inhibition of gene expression in cells and organisms. Animal RNAi studies typically involve the…
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Targeting Protein of Interest
Targeting a protein of interest is a common strategy used in drug discovery and development to develop therapeutics that specifically modulate the activity or expression of the protein of interest. There are several approaches to target a protein of interest, including small molecule inhibitors, monoclonal antibodies, RNA-based therapies, and gene editing. Small molecule inhibitors are…
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shRNA-Expressing Constructs
shRNA-expressing constructs are genetic constructs that encode short hairpin RNAs (shRNAs) for the purpose of inducing RNA interference (RNAi) in cells. These constructs are widely used in research to study gene function and to develop new therapies for a variety of diseases. It typically consist of a promoter, a shRNA sequence, and a termination sequence.…
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RNAi Stable Cell Lines
RNAi stable cell lines refer to cell lines that have been genetically modified to stably express short hairpin RNAs (shRNAs) or other RNA-based molecules that induce RNA interference (RNAi). These cell lines are used in research to study gene function and to develop new therapies for a variety of diseases. RNAi stable cell lines are…
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Transient RNAi
Transient RNAi refers to a type of RNA interference (RNAi) that results in the temporary inhibition of gene expression. RNAi is a natural biological process in which RNA molecules, such as small interfering RNAs (siRNAs), bind to specific messenger RNA (mRNA) molecules and prevent them from being translated into protein. Transient RNAi can be achieved…
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Gene Silencing Services
Gene silencing services refer to the development and production of RNA-based molecules that can inhibit the expression of specific genes. These services are used in research and drug development to study the function of genes and to develop new therapies for a variety of diseases. There are several types of RNA-based molecules that can be…